需要金幣： 1000 個金幣 |
資料包括：完整論文 |  |
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| 轉換比率：金額 X 10=金幣數量， 例100元=1000金幣 | 論文字數：10016 |  | |
| 折扣與優惠：團購最低可5折優惠 - 了解詳情 | 論文格式：Word格式(*.doc) |  |
摘要:本實驗利用枯草芽孢桿菌F7，通過固態發酵法進行抗菌肽的生產。對發酵培養基的pH、溫度、時間、固液比、碳源、氮源等營養物質進行單因素實驗研究，優化固態發酵培養基配方。最后確定培養基的最佳配方：麩皮（8g）玉米粉（2%）、豆餅粉（1%）、NaCl(0.5%)、KH2PO4（0.1%）、MgSO4?7H2O（0.04%）。采用固態發酵法，通過對指示菌金黃色葡萄球菌的抑菌效果確定最適發酵條件為：接種量1%、發酵時間96h、發酵溫度32℃、pH5、固液比1:3.5、碳源玉米粉、氮源豆餅粉，得到的抗菌肽抑菌效果為最佳。 關鍵詞 枯草芽孢桿菌；抗菌肽；發酵條件優化；抑菌效果
Abstract:This was a experiment about the production of the antimicrobial peptides. We carried out the experiment by solid state fermentation. The optimal nitrogen source, solid liquid ratio, carbon source, temperature, time, pH for the production of antimicrobial substance by bacillus subtilis were tested. The results showed that the best medium was: bran(8g)、corn powder(2%)、soybean meal(1%)、NaCl(0.5%)、KH2PO4（0.1%）、MgSO4·7H2O（0.04%）. The medium composition and fermentation conditions were optimized by solid fermentation method. The best fermentation condition was: 1% inoculum size, 96h fermentation, fermentation temperature32℃, pH 5, 1:3.5 solid liquid ratio, used corn flour as a carbon source, used soybean meal as a nitrogen source, under these conditions, we got the best inhibitory effect. Keywords Bacillus subtilis Antibiotic peptide Optimization of fermentation condition Antibacterial effect |
